US President Donald Trump signed an order on Monday that targets Iran's supreme leader and associates with additional financial sanctions.
The United States president said the action follows a series of aggressions by Iran, including last week's downing of a US unmanned drone.
Tensions between the US and Iran that have grown since May, when Washington ordered all countries to halt imports of Iranian oil.
The targets of the new sanctions include senior military figures in Iran, blocking their access to any financial assets under US jurisdiction. They also work to deny Supreme Leader Ayatollah Ali Khamenei and his close aides access to money and support.
Trump said the supreme leader and his associates were ultimately responsible for what Trump called "the hostile conduct of the regime".
"Sanctions imposed through the executive order ... will deny the Supreme Leader and the Supreme Leader's office, and those closely affiliated with him and the office, access to key financial resources and support," Trump said.
Zarif: US 'B-Team' wants war, not diplomacy
Responding to the sanctions announcement, Iranian Foreign Minister Mohammad Javad Zarif tweeted that hawkish politicians close to Trump were thirsty for war rather than diplomacy.
"@realDonaldTrump is 100% right that the US military has no business in the Persian Gulf. Removal of its forces is fully in line with interests of US and the world. But it's now clear that the #B_Team is not concerned with US interests - they despise diplomacy, and thirst for war," he tweeted.
Zarif has in the past said that a so-called "B-team" including Trump’s national security adviser John Bolton, an ardent Iran hawk, and conservative Israeli Prime Minister Benjamin Netanyahu could goad Trump into a conflict with Tehran.
Iran said earlier on Monday US cyber attacks on its military had failed, as Washington sought to rally support in the Middle East and Europe for a hardline stance that has brought it to the verge of conflict with its longtime foe.
Washington has blamed Tehran for attacks on tankers in the Gulf in recent weeks, which Iran denies. On Monday, the US said it was building a coalition with allies to protect Gulf shipping lanes.
programme, a senior US State Department official said, without identifying the countries.
"It's about proactive deterrence, because the Iranians just want to go out and do what they want to do and say hey we didn't do it. We know what they've done," the official told reporters, adding that the deterrents would include cameras, binoculars and ships.
The US accuses Iran of encouraging allies in Yemen to attack Saudi targets.
In a joint statement on Monday, the US, Saudi Arabia, the UAE and Britain expressed concern over Middle East tensions and the dangers posed by Iranian "destabilising activity" to peace and security in Yemen and the region.
The confrontation between Iran and the US heated up last Thursday when Iran shot down a US drone, saying it had flown over its airspace.
Washington, which said the drone was in international skies, then appeared to come close to attacking Iranian military targets, with Trump saying that he aborted a retaliatory air attack 10 minutes before it was to go ahead.
Trump said he decided the attack would have possibly killed too many people.
US media have reported that Washington launched cyber attacks last week even as Trump called off his air strike. The Washington Post said on Saturday that the cyber strikes, which had been planned previously, had disabled Iranian rocket launch systems. US officials have declined to comment.
Iran dismissed the cyber attacks as a failure.
"They try hard, but have not carried out a successful attack," Mohammad Javad Azari Jahromi, Iran's minister for information and communications technology, said on Twitter.
"Media asked if the claimed cyber attacks against Iran are true," he said. "Last year we neutralised 33 million attacks with the (national) firewall."
Fears of war
US allies have been calling for steps to defuse the crisis, saying they fear a small mistake by either side could trigger war.
"We are very concerned. We don't think either side wants a war, but we are very concerned that we could get into an accidental war and we are doing everything we can to ratchet things down," British Foreign Secretary Jeremy Hunt said.
US Secretary of State Mike Pompeo jetted to the Middle East to discuss Iran with the leaders of Saudi Arabia and the United Arab Emirates. Pompeo met King Salman as well as the king's son, de facto ruler Crown Prince Mohammed bin Salman.
The US special representative for Iran, Brian Hook, visited Oman and was headed to Europe to explain US policy to allies. He told European reporters on a phone call ahead of his arrival that Trump was willing to sit down with Iran, but that Iran must do a deal before sanctions could be lifted.
US-Iran relations have deteriorated over the past year since the US abandoned a 2015 agreement between Iran and world powers designed to curb Iran's nuclear programme in return for the lifting of sanctions.
US allies in Europe and Asia view Trump's decision to abandon the nuclear deal as a mistake.
France, Britain and Germany have sent an official diplomatic warning to Iran if Tehran reduces its compliance with the accord, two European diplomats said on Monday.
It was not immediately clear what consequences Iran might face for non-compliance.
Washington argues that the agreement known as the JCPOA, negotiated under Trump's predecessor, President Barack Obama, did not go far enough, and new sanctions are needed to force Iran back to the table to make more concessions.
Both sides have suggested they are willing to hold talks while demanding the other side move first. In the latest comment from Tehran, an adviser to Rouhani repeated a long-standing demand that Washington lift sanctions before any talks.
But the adviser, Hesameddin Ashena, also tweeted a rare suggestion that Iran could be willing to discuss new concessions, if Washington were willing to put new incentives on the table that go beyond those in the deal.
"If they want something beyond the JCPOA, they should offer something beyond the JCPOA; with international guarantees," he said.-Al Jazeera